82 {X4*!. Mycologia 



made of the same material on S. lobata, C. digitata, Hibiscus 

 militaris, and Malvastrum coccineum, with infection only on the 

 Sphaeralcea, pycnia showing April 6, but the aecia being destroyed 

 by aphis before maturing. 



Another collection of identical appearance was made five days 

 later than the one above, at Ysleta, Texas, a few miles from El 

 Paso. This was sown, March 28, on two plants of 5*. lobata, on 

 C. digitata, and H. militaris. Both plants of Sphaeralcea gave in- 

 fection, showing pycnia April 6, but resulting in few aecia, owing 

 to accident. Another sowing from the same collection was made, 

 May 2, on Napaea dioica, without infection. 



Putting the above cultural results from P. tosta, with the gross 

 appearance and subsequent microscopic study, and comparing with 

 similar data from P. Muhlenbergiae, there appears to be no 

 ground upon which to keep the two forms separate. The fact that 

 the telial hosts belong to two genera of grasses is not in this case 

 a difference of importance, as Sporobohis and Muhlenbergia in- 

 tergrade, and are kept separate upon technical grounds. The 

 previous cultural studies 35 of P. Muhlenbergiae have indicated 

 that the species is a complex of races. It has been grown on 

 Hibiscus militaris and Callirhoe involucrata, but the similar aecia 

 on Napaea, Malvastrum, and Sidalcea have not yet been grown, 

 although attempted, the failure believed to be due to a lack of the 

 proper racial telia. The above is the first successful culture with 

 material on Sporobohis, although previous attempts 36 have been 

 made. 



Hereafter it will be considered advisable to treat P. tosta 

 as a synonym of P. Muhlenbergiae. 



21. Puccinia Agropyri E. & E. A collection of this rust on 

 Elymus virginicus was made by Dr. Fromme and the writer on 

 Feb. 13, 19 14, at Austin, Texas, and at the same time the observa- 

 tion was made that Clematis Drummondii, not then in leaf, was 

 in the same spot, and abundant in the vicinity. Dormant roots 

 of this wild Clematis were secured, and later grown for the culture 

 work. 



35 See Jour. Myc. 11: 51. 1905; 13: 192. 1907; Mycol. 1: 251. 1909; 2: 

 226. 1910; and 4: 18. 1912. 



36 See Jour. Myc. 10: 10. 1904; 12: 12. 1906; Mycol. 4: 10 and 52. 

 191 2. 



