Arthur: Cultures of Uredineae S>d/1 85 



244 



Cultures in 1913 



1. Uromyces elegans (B. & C.) Lagerh. This autoecious 

 rust, very common in the Southern States, is one of the group 

 of species which produce no uredinia, so far as known, very few 

 of which have been cultivated. Growing plants of Trifolium Car- 

 olinian urn bearing aecia were sent by Dr. F. A. Wolf from 

 Auburn, Ala., and the aeciospores sown March 29 on plants free 

 from the fungus. Teliospores began to appear about April 18 

 as the result. 



This result does not disclose whether the species possesses both 

 primary and secondary aecia, or whether pycnia ever occur. A 

 culture with teliospores should be kept in view. 



Cultures in 1914 



2. Puccinia nodosa Ell. & Hark. While upon a visit to the 

 Desert Botanical Laboratory at Tucson, Ariz., an exceptional op- 

 portunity was offered to study the life history of another autoe- 

 cious species of rust, which like the one last mentioned, possesses 

 no uredinia. A strong plant of Brodiaea pauciflora, growing near 

 the door of the laboratory was moistened on February 26, sprinkled 

 with aeciospores brought from some distance, and covered with a 

 belljar. The temperature at the time was favorable for infection, 

 the belljar being shielded from the direct rays of the sun. The 

 experiment was left in charge of Dr. W. A. Cannon of the Labo- 

 ratory staff, who kindly forwarded the leaves on March 18, when 

 the sori first opened. Only telia were produced. 



As in the preceding case the possibility of both primary and 

 secondary aecia occurring in the life cycle is not touched upon. 



3. Puccinia splendens Vize. Through the courtesy of the 

 Desert Botanical Laboratory, Dr. Fromme and the writer were 

 enabled to make an excursion on February 28 to the Santa Rita 

 Mountains in the vicinity of Tucson, Ariz., where we secured 

 teliosporic specimens of P. splendens on Hymenoclea tnonogyra. 

 Through the kindness of Prof. J. J. Thornber of the University 

 of Arizona we received after our return to Indiana a number of 

 thrifty young plants of this host, which soon started into leaf in 

 the green house. A sowing of teliospores was made April 7, 



