251 



Arthur: Cultures of Uredineae in i$r<, ~ 127 



was sown March 31, on Smilax hispida; another collection from 

 the same locality, made May 16, 191 5, was sown on another plant 

 of the same host May 24, both without infection. The leave? 

 of the two collections sent by Mr. Bethel were thickly covered 

 with the prominent, blackish telia. The teliospores germinated 

 well, especially so in the October collection. Mr. Bethel stated 

 on the label of the latter specimen that although the rust was very 

 abundant, there were no Smilax plants in the region. In sending 

 the May collection he stated on the label that the aecial stage oc- 

 curred on Leucocrinum, but desired to have the material tested on 

 Smilax. In the accompanying letter of same date he says: "I 

 am inclosing Puccinia amphigena on Calamovilfa longifolia, which 

 I wish you would sow on Smilax, which I believe you give as host 

 in the east. I have fine young plants of native Smilax, but have 

 failed to inoculate them. With us this does not normally belong 

 to Smilax, and may be another race. It belongs to Leucocrinum, 

 on which it is epidemic everywhere this year." This species of 

 rust was first cultured on Smilax in 1902, from telial material 

 gathered at Callaway, Neb., and in the year following from ma- 

 terial gathered at the type locality in Chicago, 111. It has been 

 cultured altogether six times, 3 the westernmost locality for telial 

 material being central Nebraska, on the plains, some two hundred 

 miles from the foothills of the Rocky Mountains, where this 

 season's material was obtained. 



2. Puccinia emaculata Schw., on Panicum capillare L., col- 

 lected at Lafayette, Ind., was sown, May 21, on Euphorbia corol- 

 lata, having shown strong germination, but without results. The 

 reasons for this attempt, with citation of other trials, are given in 

 the previous report of cultures. 4 



3. Puccinia simillima Arth., on Phragmitcs communis Trim, 

 collected by Dr. J. F. Brenckle, at Wiedmer's Lake, N. Dak., on 

 April 11, 1915, was sown May 14, on Anemone cylindrica and 

 Ranunculus acris, without infection. It was sown again June 18 

 on two plants of Ranunculus scclcratus, but with no infection. 



3 See Bot. Gaz. 35:20. 1903; Jour. Myc. 10:11. 1904; 12:16. 1906; 

 14: 15. 1908; Mycol. 2:225. 1910; and 4: 18. 1912. 

 * Mycol. 7 : 65. 1915. 



