Introduction 



This report evaluates the biological integrity', support of aquatic life uses, and probable 

 causes of stress or impairment to aquatic communities at 30 sites on 19 streams in the Blackfoot 

 River TMDL Planning Area of west central Montana. The purpose of this report is to provide 

 information that will help the State of Montana determine whether these streams are water- 

 quality limited and in need of TMDLs. 



The federal Clean Water Act directs states to develop water pollution control plans (Total 

 Maximum Daily Loads or TMDLs) that set limits on pollution loading to water-quality limited 

 waters. Water-quality limited waters are lakes and stream segments that do not meet water- 

 quality standards, that is, that do not fully support their beneficial uses. The Clean Water Act 

 and USEPA regulations require each state to (1) identify waters that are water-quality limited, 

 (2) prioritize and target waters for TMDLs, and (3) develop TMDL plans to attain and maintain 

 water-quality standards for all water-quality limited waters. 



Evaluation of aquatic life use support in this report is based on the species composition 

 and structure of periphyton (a.k.a. benthic algae, phytobenthos) communities at 30 sites on 19 

 streams that were sampled in September and October of 2003. Periphyton is a diverse 

 assortment of simple photosynthetic organisms called algae that live attached to or in close 

 proximity of the stream bottom. Some algae form long filaments or large gelatinous colonies 

 that are conspicuous to the unaided eye. However, most algae, including the ubiquitous diatoms, 

 can be seen and identified only with the aid of a microscope. The periphyton community is a 

 basic biological component of all aquatic ecosystems. Periphyton accounts for much of the 

 primary production and biological diversity in Montana streams (Bahls et al. 1992). Plafkin et 

 al. (1989) and Barbour et al. (1999) list several advantages of using periphyton in biological 

 assessments. 



Biological integrity is defined as "the ability of an aquatic ecosystem to support and maintain a balanced, 

 integrated, adaptive community of organisms having a species composition, diversity, and functional organization 

 comparable to that of natural habitats within a region" (Karr and Dudley 1981). 



