PHYSICOCHEMICAL PROPERTIES 41 



Experiments with doses of arsenic made by Mile. Michel 

 (by the method of Bougault) have given the following 

 results. 



EXPERIMENT 1. The whole blood of a rabbit bled white 

 is added to 10 eg. of luargol in a solution of 10 c.c. of distilled 

 water, is shaken violently to prevent coagulation and allowed 

 to stand for twenty-four hours. The fluid is centrifuged and 

 38 c.c. of dark liquid and 30 c.c. of precipitate are obtained 

 in which are found : 



Arsenic, mg. 



In the fluid 4.62 



In the precipitate 15.37 



in all the same 20 mg. of arsenic contained in the 10 eg. of 

 luargol of which about one-quarter remains in solution in 

 the serum and the other three-quarters have been partly 

 fixed by the formed elements of the blood and partly pre- 

 cipitated as finely granular viscous masses. Only a very 

 small quantity of coagulated fibrin is formed. 



EXPERIMENT 2. A rabbit is injected intravenously with 

 10 eg. of luargol dissolved in 10 c.c. of distilled water and is 

 bled twenty-four hours afterward. At the same time the 

 urine for the twenty-four hours is collected and is added to 

 the feces as well as to the intestinal contents after the bleed- 

 ing. The blood is centrifuged and we find : 



In 55 c.c. of coagulum about 1 . mg. of arsenic \ Q R 



In 37 c.c. of serum 2.5 " " /* 



In 275 c.c. of urine 2.3 " " \ . , 



In 90 gr. of fecal matter 3.2" / 



which means that a little more than a quarter of the injected 

 luargol has been eliminated in twenty-four hours by the 

 kidneys and intestines; about one-eighth remains in the 

 serum as un transformed luargol and one-sixteenth has been 

 absorbed and fixed in the elements of the circulating blood at 

 the moment of bleeding. In estimating at 10 per cent, the 

 quantity of blood remaining in the vessels, we may assume 

 that after twenty-four hours approximately one-half of the 

 luargol originally injected has remained fixed in the organs 

 or tissues. 



