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two live vaccines, and inoculated, first, myself and 

 a number of my personal friends, so that the 

 reaction produced by the injection could be care- 

 fully observed, and its harmlessness established. 

 Then I extended the operation to 42,197 persons 

 inhabiting ninety-eight different localities in India 

 -viz., in Bengal, Behar, the then North- Western 

 Provinces and Oudh, the Punjab, the Brahmaputra 

 Valley, and Lower Assam. In 1895-96 I inoculated 

 a further 30,000 people in Bengal, Behar, Assam, 

 the Central Provinces, and the Bombay Presidency. 

 It was necessary to spread the operations in this 

 manner, as it was not known exactly where cholera 

 outbreaks might occur; while in some of the localities 

 most threatened with such outbreaks namely, in 

 Bengal, where I carried out subsequently the most 

 instructive of my operations I was unable, for a 

 considerable time, to obtain assent to my work. 

 My efforts were directed to inoculating people 

 under such conditions as would afterwards render 

 possible an accurate study of results. In this 

 manner I inoculated part of the officers, non-com- 

 missioned officers, and men in 64 British and native 

 regiments ; a proportion of the coolie population 

 in 45 Tea Estates in Assam, Cachar, Sylhet, and 

 the Chittagong district ; part of the inmates of 

 boarding-schools and orphanages, and of 9 civil 

 jails ; the population of a supervised village of 

 Sansis (one of the criminal tribes) near Sialkot, 

 Punjab ; inhabitants of Himalayan villages situated 

 along the Hardwar pilgrim route, between Nairn 

 Tal and Mussoorie, and liable to become infected 

 with cholera ; residents of the suburban quarters 

 (bustees] of Calcutta, and so on. Elaborate arrange- 

 ments were made among these communities for 



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