858 CYCLOPEDIA OF LIVE STOCK AND COMPLETE STOCK DOCTOR. 



1. That the tuberculin test is a wonderfully accurate method of deter- 

 mining whether an animal is affected with tuberculosis. 



2. That by the use of tuberculin the animals diseased with tuberculosis 

 may be detected and removed from the herd, thereby eradicating the 



3. That tuberculin has no injurious effect upon healthy cattle, 



4. That the comparatively small number of cattle which have aborted, 

 suffered in health, or fallen off in condition after the tuberculin test were 

 either diseased before the test was made or were affected by some cause 

 other than the tuberculin. 



VII. Summary of Directions for Making the Tuberculin Test. 



1. Stable cattle under usual conditions and among usual surround- 

 ings, feeding and watering in the customary manner. 



2. Make a physical examination of each animal, and give to each 

 one some designation by which the animal will be known throughout 

 the test. 



3. Take each animal's temperature at least three times at two or three 

 hour intervals on the day of injection; for instance, at 2, 5, and 8 p. m. 



4. At 8 or 10 p. m. inject a dose of tuberculin under the skin in the 

 region of the shoulder, using a sterile hypodermic syringe after dis- 

 infecting the skin at the seat of injection with a 5 per cent solution of 

 carbolic acid or a similar antiseptic solution. 



5. Tuberculin is not ahvays concentrated to the same degree, and 

 therefore the dose, which should always appear on the label, varies con- 

 siderably. The dose of imported tuberculin is 0.25 c. c. for an adult 

 cow, and before injection is diluted with sterile water to 2 c. c. The 

 tuberculin made by the Bureau of Animal Industry is prepared so that 

 it will not be necessary to dilute it, and the dose is 2 c. c. for an adult 

 animal. Yearlings and 2-year-olds, according to size, should receive 

 from 1 to li/> c. c, while bulls and very large animals may receive 3 c. c. 



6. At 6 a. m. on the day following the injection of tuberculin com- 

 mence taking temperatures, and continue every two or three hours until 

 the twentieth hour after injection, at which time if there is no tendency 

 for the temperature to rise the test may cease. 



7. A rise of 2° F. or more above the maximum temperature observed 

 on the previous day, providing the temperature after injection exceeds 

 103.8° F., should be regarded as an indication of tuberculosis. Those 

 cavses which approximate but do not reach this standard should be con- 

 sidered as suspicious and held for a re test six weeks later, giving double 

 the original dose. 



