Paraffin Method 



21 

 Living tissue 



( 



Fixing 



( i-) 



Washing 



i 

 Alcohols ( 9) 



(50%, 67%, 82%)<- 



Dehydration 

 (95%-99% alcohol) 



-Staining in toto 

 (79) 



Celloidin Method 



Toluene, 1 part 

 Abs. Alcohol, 1 part. 

 (Ihr. to 1 day) 



1 

 Pure toluene, 



(1 hr. to 1 day) 



i 



Toluene, 1 part, 

 Paraffin, 1 part. 

 (2 hrs. to 2 days) 



i 



Pure Paraffin 



(2 -to 24 hrs.) 



Clearing 



Ether-alcohol 

 days) 



. . Infiltration 



Imbedding paraffin, 



mass cooled. Imbedding. 



Paraffin sections 

 cut. 



Sectioning 



Thin celloidin 



(2% solution) 



(1-14 days) 



f: 



Thick celloidin 

 (6% solution) 

 (1-14 days) 



1 

 Thick celloidin 



(12% solution) 

 (1-14 days) 



! - 



Celloidin mass 

 hardened in 

 chloroform 



! 



Celloidin block 

 clarified or 

 placed in 82% 

 alcohol 



Celloidin sections 

 . .cut 



