39 



89. Mayer's Haemalum. Formula: haematein, 1 gram; 90% (95%) 

 alcohol, 50 c. c.; potash alum, 50 grams; distilled water, 1,000 c. c. Dissolve 

 the haematein in the alcohol, the alum in the water; mix. 



This is an excellent formula, giving a good stain immediately after it is made 

 up and retaining its selective staining quality for a year or longer. It is one of the 

 best hematoxylins for ordinary work. 



For this formula, Mayer has now substituted the following: hematoxylin, 

 1 gram (10 cc. stock solution); distilled water, 1,000 c. c.; sodium iodate, 0.2 

 grams; potassium alum, 50 grams. The hematoxylin is first dissolved in the 

 water and then the other ingredients are added. Dissolve and filter. 50 grams 

 of chloral hydrate and 1 gram of citric or acetic acid may be added as a preserva- 

 tive. Stain as given in 88. In using it is frequently advisable to dilute one or 

 more times with distilled \vater. 



90. Ehrlich's acid hematoxylin. Formula: Water, 100 c. c.; 95% 

 alcohol, 100 c. c. hematoxylin crystals, 2 grams (20 c. c. stock solution) ; dissolve in 

 the alcohol; glycerin, 100 c. c.; glacial acetic acid, 10 c. c.; alum in excess. Let 

 the mixture ripen in the light until a dark red. Sections stain in this hematoxylin 

 in a short time, generally 5 to 10 minutes. Wash with water after staining. 



91. Delafield's hematoxylin. Formula: Saturated aqueous solution 

 of ammonia alum, 200 c. c.; hematoxylin .stock solution 20 c. c. Allow the 

 mixture to stand in the light and air in an unstoppered bottle for 4 or 5 days; 

 filter and add glycerin, 50 c. c., and methyl alcohol, 50 c. c. Permit it to stand 

 for a week or so to ripen; filter and keep in a stoppered bottle. The staining 

 power increases for several months. In using, dilute 3 or 4 times or more with 

 distilled water. It is useful for in toto staining diluted 1 :9 with 20% alcohol or 

 distilled water. 



Stain sections from water; 4 to 5 minutes will generally be sufficient. Wash 

 well with water after staining. In toto staining 1 to 14 days may be necessary 

 depending upon the size of the object. 



This is a very strong hematoxylin stain and may be used to advantage with 

 tissues that stain with difficulty. It is likewise a more diffuse stain than either 

 chloral or Ehrlich's hematoxylin, staining cell-body as well as nucleus, a feature 

 having its advantages. Old solutions (several months to a year) should be 

 filtered before using. 



92. Acid Hematoxylin (Delafield's). The dilution of Delafield's hema- 

 toxylin ten to twenty times with water, and making slightly acid with acetic 

 gives a useful stain particularly for differentiating nuclei (embryological work.) 



93. Muchematein (Mayer). Aqeuous formula: Rub up 0.2 grm. hema- 

 tein with a few drops of glycerin; add 0.1 grm. aluminium chlorid, 40 c. c. glycerin, 

 60 c. c. distilled water. Filter if necessary. Alcoholic formula: Hematein, 0.2 

 grms.; aluminium chlorid, 0.1 grm.; 70% (67%) alcohol, 100 c. c.; 1 or 2 drops 

 nitric acid. Formulae with a minimum amount of aluminium designed as specific 

 stain for mucus. The aqueous solution is more selective; the alcoholic formula 

 designed for the staining of mucus that swells excessively in water ( 234). 



94. Iron Hematoxylin (Heidenhain). Formula: (a) Mordant- 2% 

 aqueous solution of ferric alum (iron-ammonium-persulphate), (b) Stain; 

 aqueous solution of hematoxylin (10% alcoholic stock solution, 5 c. c.; 



