46 



Stain sections from alcohol, 12 to 24 hours; wash in water 1 to 5 minutes; 

 differentiate in a 1% ferric chlorid solution (ale. stock solution, 1 part, water 6 

 parts). Counter stain with picric acid (116). A sharp stain, though possibly 

 not in all cases completely differential. 



128. Jenner's Stain [27]. This is an eosinate of methylene blue. Formula: 

 Mix equal parts of 1.25% eosin (water soluble) and 1% methylene blue. Permit 

 the mixture to stand for 24 hours, then filter, wash the precipitate on the filter 

 with distilled water, dry it and dissolve it in methyl alcohol (0.5 grm. precipitate 

 in 100 c. c. alcohol). 



This is primarily a stain for blood films. Sections may be stained in one of two 

 ways: (a) Stain 1 to 5 minutes, dilute the stain with an equal volume of water 

 and continue the staining for 5 to 10 minutes. Rinse with distilled water, differ- 

 entiate and dehydrate rapidly with 95% and absolute alcohol. Clear in xylene. 

 Mount in balsam. 



(b) Dilute the stain with 1 to 4 volumes of distilled water and stain 1 to 24 

 hours. Further treatment as above. To insure permanency, it is well to thor- 

 oughly remove the alcohol with xylene in clearing, by 2 or 3 treatments. 



129. Wright's stain. Contains several staining principles, notably eosin- 

 ates of methylene blue, methylene azure, in solution in methyl alcohol, and is 

 made according to a complicated method, for which see original article [46]. 



Primarily for blood films ( 214). Dissolve 0.2 grams of the dry stain in 100 

 c. c. of methyl alcohol. In staining sections follow the procedure given in 128. 



130. Nochts-Hastings Stain [22]. Similar in its composition to Wright's 

 stain and used in a similar manner. In staining sections, follow the procedure given 

 in 128. Dissolve 0.3 grams of the dry stain in 100 c. c. of methyl alcohol. An 

 excellent blood stain ( 214). 



131. Neutral Gentian Violet (Reinke, Bensley). Formula: Saturated 

 aqueous solution of gentian violet and saturated solution of orange G. are mixed 

 in equal proportions and permitted to stand 24 hours. The precipitate that forms 

 is removed by filtration, washed with distilled water, dried, and a concentrated 

 solution in methyl or ethyl alcohol is made. When ready to use it, dilute 1 to 4 

 times with distilled water. Stain 12 to 24 hours, drain away the stain, dehydrate 

 rapidly with 95% and absolute alcohol, continuing the differentiation if necessary 

 with oil of cloves, to the right degree. Remove the oil of cloves with xylene 

 (thoroughly) and mount in balsam. 



PREPARATION FOR STAINING. 



132. After the sections are cut and before the process of 

 staining can begin, certain steps are necessary, such as the removal 

 of the paraffin in the case of paraffin sections. Furthermore, it is 

 usually advisable to fasten the delicate sections to the glass slides on 

 which they are to be finally mounted before beginning the series of 

 manipulations that are necessary in Staining and Mounting. To 

 consider the latter first: 



