6 



necessary to make a study of the particular technical needs of the 

 problem, and for this it is well to consult the larger works on technique 

 of which may be mentioned the Encyclopedia of Microscopical 

 Technique [6]; The Microtomist's Vade-mecum by A. B. Lee [30]; 

 Physiological Histology, by Gustav Mann [36]; Mallory & Wright, 

 Pathological Technique [35]. The books of Gage, Hardesty and v. 

 Kahlden-Gierke will also be found valuable for consultation. 



General Histological Technique involves then : 



A. Examining fresh, by either B. or C. Advantageous or necessary 



when haste is required, or in examining the tissue alive. 



B. Isolation or Dissociation. Separating out the elements composing 



a tissue by (a) teasing or (b) treatment with reagents and 

 teasing. 



C. Cutting thin sections of the tissue or organ. 



For C. are generally necessary: . 

 1. Fixing the tissue ( 1 34). Hardening. 

 %. Sectioning by one of the following methods : 



(a) Free-hand, without an imbedding mass, or 



(b) With an imbedding mass, as 



(1) By the Paraffin method ( 50) , or 



(2) By the Celloidin method ( 61) , or 



(3) By the Freezing method ( 73) . 



D. Staining; to outline and differentiate the structure, or pick 



out definite chemical substances. 



E. Mounting; for examination under the microscope and per- 



manent preservation. 

 In addition, numerous 



F. Special Methods and methods for the 



G. Histo-chemical Analysis of structure must be frequently applied. 



