53 



(a) When the preparation is on the cover. Prepare a shellac 

 cell ( 156) on the slide of a size slightly smaller than the cover to 

 be used, and allow it to dry for a day or so. Warm the cover bear- 

 ing the preparation to remove the last traces of moisture, and place 

 it film side down upon the ring. Warm the slide until the edge of 

 the cover may be made to adhere to the shellac ring and press the 

 cover down until it adheres all the way round. Seal the cover with 

 shellac and label ( 163, 165). 



(b) Mounting in a paper or rubber cell. With a brush, cover 

 one side of the ring with a layer of shellac and place it on the center 

 of the slide, shellac side down; place within the cell the prepara- 

 tion, arranging it in the manner desired, and place upon the ring a 

 cover-glass of a suitable size, and seal it with shellac; label. 



151. Mounting in glycerin media, (a) Pure glycerin; (b) 

 glycerin and acetic acid, 1%; (c) glycerin and a stain. As glycerin 

 extracts most stains (Ex. Carmine) it is sometimes advisable to 

 have a small amount of stain dissolved in the glycerin used for 

 mounting. Of such combined mounting and staining mixtures may 

 be mentioned (1) Glycerin and Congo red, (2) Glycerin and car- 

 mine. Other combinations may be used. 



152. Congo Glycerin. Formula: J/2% aqueous solution, 

 Congo Red, 1 part; glycerin, 1 part. 



153. Carmine Glycerin. Formula: Carmalum, 25 c. c., 

 Glycerin, 75 c. c. 



154. Methyl green and Eosin Glycerin. Formula: 1% 

 aqueous solution methyl green, 2 c. c.; >% aqueous eosin, 1 c. c., 

 glycerin, 97 c. c. 



Glycerin and glycerin-jelly are most serviceable in mounting 

 isolation preparations. For both of these mounting media the ob- 

 ject must be mounted from water or an aqueous solution. 



Arrange the section or teased tissue in the center of the slide, 

 drain off the water or aqueous solution in which the preparation is 

 and add a small drop of glycerin. Take a clean cover in the forceps, 

 breathe on the under side and carefully lower it upon the object; 

 gently press it down. It is best to use only a small drop of glycerin 

 so as not to get it outside the cover, as it is hard to clean 

 away satisfactorily. Clean carefully and seal with shellac in 

 accordance with 163. 



