94 



Dissociation, dissociators, 6, 16- 

 Double staining, double stains, 37 

 Dry mounting, 52 

 Ehrlich's acid hematoxylin, 39 

 Ehrlich-Biondi-Heidenhain triple stain, 



42 



Elastic fibers (elastin), stains for, 45, 62 

 Eosin, 43 



Eosin-methylene blue, 42 

 Eosinate of methylene blue, 46 

 Epithelium, silvering, 83 



isolation (dissociation) of, 17, 1 8 

 Erlicki's fluid, 15 

 Erythrosin, 43 

 Ether-alcohol, 27 

 Euparal, 55 



Fastening sections to slide, 47- 

 Fat, fixation of, 72, 84 



freezing microtome, use, 84 



stains for, 84 

 Fixation, 7, 8 

 Fixation by injection, 9 

 Fixation, rules for, 9 

 Fixers, action of, 7 



formulae for, 11-16 



list of, 8 

 Fixing, 6, 7 



Flemming's fluid, 13, 74, 85 

 Flemming's triple stain, 43 

 Formaldehyde, formalin, 14, 15 



Dissociator, 18, 68 

 Formol-dichromate, 12 

 Free- (hand) sections, 47, 48 

 Freezing method for sectioning, 19, 33, 



48,84 



Fresh tissue, examination of, 6 

 Fuchsin acid, 44 

 Gage's dissociators, 18 

 Gage's hematoxylin, 38 

 Gage's method for glycogen, 86 

 Gelatin masses, 82 

 Gentian violet, 41, 43, 46, 87, 88 

 Gilson's fluid; see mer euro-nitric 

 Glycerin, 53, 55 

 Glycerin- jelly, 54, 56 

 Glycogen, fixation, staining, 86 

 Gold chlorid, methods, 69 

 Golgi methods, 74 

 Gram's solution; see iodine, 45 

 Granules, cytoplasmic, 60 



secretion, 61 

 Ground sections, 64 

 Gum arabic, 33 

 Hsemalum, Mayer's, 39 

 Hair, dissociation of, 18, 19 

 Hardening of tissues, 10 



celloidin mass, 29 

 Hasting's stain, 46, 81 

 Heidenhain's iron hematoxylin, 39 

 Held's method (nerve cells) , 70 



Kelly's fluid, 12 

 Hemateates, hematein, 38 

 Hematoxylin stains, formulae, 38, 39, 40 



stock solution, 38 

 Hermann's fluid, 13 

 Herxheimer's stain, 85 

 Horn, dissociation of, 18, 19 

 Hydrochloric acid decalcifier, 63 

 Hydrochloric acid carmine, 41 

 Imbedding, celloidin, methods, 20, 26 



paraffin, 20, 22 

 Impregnations, 36 

 Indifferent fluids, 16 

 Indophenol, 85 

 Infiltration, (imbedding methods), 22, 



26, 33 



Injection, fine, dense, masses, 82, 83 

 In toto staining, 35, 41 , 50 

 Intra vitam methylene blue, 76 

 lodin, ii, 45, 86, 87 

 Iron hematoxylin, Heidenhain's, 39 

 Iron, tests for, 88, 89 

 Isolation ,16-, 66, 68, 69 

 Jenner's stain, 46, 8 1 

 Labeling slides, 56 

 Lampblack, gelatin mass, 83 

 Light green, 45 

 Lipoids ; see fats 

 Lipoids; foot note, 7 



demonstration, 84 

 Lugol's solution; see iodine, 45 

 Lyon's blue, stain; see Bleu de Lyon, 



45 



Maceration, 16 



Mallory's connective tissue stain, 44 

 Marchi methods (degenerating nerve 



fibers), 73 



Mayer: see carmalum, haemalum 

 mucicarmine, Muchematein, par- 

 acarmine 



Mercuric chlorid, 1 1 

 Mercuro-nitric, 13 

 Methylene blue, 42, 70, 76 

 Methyl green, 42 

 Micro-chemical tests, 58 

 Microtome knife, care of, 58 

 Mitochondria, fixation and staining, 60, 



61 



Mordantage, 35 

 Mounting media, methods, 6, 52- 



cells, 53, 54 

 Muchematein, 39, 88 

 Mucicarmine, 41, 88 

 Mucus, 87 

 Miiller's fluid, 15 



dissociator, 17 

 Muscle, isolation of, 66 

 Muscle, methods for, 66- 

 Myelin, myelinic nerve fibers, 69 

 Nail, dissociation of, 18, 19 



