95 



Nerve cells, isolation, staining, 68 

 Nerve fibers, isolation, degenerated, 



69,73 



Nervous system, methods, 68- 

 Neurofibrillae, 71 

 Neuroglia, stain for, 79 

 Neutral gentian, 46 

 Neutral stains, 37, 35, 36 

 Nissl's methylene blue, nethod, 76 

 Nitric acid, decalcifier, dissociator, 



fixer, 14, 1 6, 63 

 Nochts-Hastings stain, 46, 81 

 Normal, salt solution; see physiological 



salt solution 

 Orange-fuchsin acid, 62 

 Orange G., 44 

 Orcein, 45, 62 



Orth's fluid; see formol-dichromate 

 Osmic acid, dissociator, fixer, 15, 18, 69, 



73, 75 



Osmium tetroxid ; see osmic acid 

 Pal's method, 73 

 Paracarmine, 41 



Paraffin, grades, method, 20, 21-, 23 

 Paraffin sections, handling of, 48 

 Per enyi's fluid, 14 

 Peroxid, as bleacher; see Hermann's 



fluid 



Phosphorus, 89 



Physiological salt solutions, 16 

 Picric acid, stain, 44 

 Picro-aceto-formol, 12 

 Picro-fuchsin, 44, 62 

 Picro-nitric, 14 

 Platino-aceto-osmic : see Hermann's 



fluid 



Potassium dichromate, fixer, 16 

 Potassium hydroxid dissociator, 19, 67 

 Potassium, tests for, 89 

 Progressive staining, 35 

 Pyridin; see Cajal's silver methods, 71 

 Pyroxylin; see celloidin 

 Quadruple stains, 89 

 Radium, to prevent electric sections, 26 

 Regressive staining, 35 

 Resorcin fuchsin, 45, 62 

 Reticular tissue, 62 

 Safranin, 42, 85 

 Scarlet red, 85 

 Sealing preparations, 55 



Secretion granules, 61 

 Sectioning, methods of, 6, 19- 

 Section staining, 35 

 Serial sectioning in celloidin, 32 

 Shellac, cells, rings, 54 



sealing, 55, 56 

 Silvering blood vessels, 83 

 Silver nitrate, 83, 71, 74 

 Slides, cleaning, 57 

 Soluble cotton ; see celloidin 

 vStaining, forms of, rules of, 35 

 Staining, isolated cells, sections, etc., 



18, 51, 53, 66, 67 

 Stains, 35-, 38 

 Stock solutions, 1 1 

 Storing of tissues, 10 

 Substantive staining, 35 

 Sudan III, 85 



Sulphalizarinate of sodium, 61 , 79 

 Sulphuric acid dissociator, 18 

 Tellesnicky's fluid; see dichromate 



acetic 



Tigroid substance, 70 

 Tissues, fixing, 6, 7 - 



hardening, storing, 10 

 Tissue paper, for handling sections, 31 

 Toluene, as clearer, 22, 21 

 Toluidin blue, 42 

 Tooth, 63, 64 



Transferring celloidin sections, 31 

 Triple staining, 37 

 Triple stain; Ehrlich-Biondi, 42 



Flemming's, 43 



Ultramarine, gelatin mass, 83 

 van Gehuchten's, 74 

 Vascular epithelium, silvering, 83 

 Verhceff's elastin stain, 45 

 von Luko's fluid; see alcohol-aceto- 



formol 



Weigert methods, for nerve, 71 

 Weigert's copper hematoxylin, 40, 71 

 White connective tissue fibers, 61 

 Wooden blocks, for celloidin imbed- 

 ding, 28, 30 

 Wright's stain, 46, 81 

 Xylene, 23, 50 

 Xylene, as clearer, 22, 23 

 Xylol; see Xylene, 22 footnote 

 Zenker's fluid, 1 1 

 Zenker-formol; see Helly's fluid, 12 



PRESS OF W. F. HUMPHREY, GENEVA, N. Y. 



