SINGLE NATURE OF HEMOLYTIC IMMUNE BODIES. 335 



TABLE I. 



Red blood corpuscles of ox per c.mm. , 5,880,000. Red blood corpuscles of rabbit per c.mm., 



4,240,000. 



As regards the technic of hemolytic experiments, it may be well 

 to note the role played by an excessive amount of isotonic salt 

 solution in the suspensions of test corpuscles employed by many 

 observers. It would seem, a priori, desirable to deal with cor- 

 puscles as nearly as possible in their normal condition of suspension 

 in the blood, but the advantages of a 5 per cent suspension are 

 manifest, since smaller amounts can be accurately measured and a 

 great waste of often precious materials obviated. But I have noted 

 repeatedly that the dilution of a small amount of alexic serum by 

 so great an excess of isotonic solution unquestionably affects its 

 activity and often completely inhibits it. This is well shown in the 

 case of sensitized rabbit corpuscles when acted on by rabbit alexin. 



TABLE II. 



