202 



COLLECTED STUDIES IN IMMUNITY. 



functions. We were able to observe this behavior repeatedly and 

 reproduce the following as an illustration. 



10 cc. goat serum are shaken with 8 cc. rabbit blood for a very 

 short time and then rapidly centrifuged. The following table shows 

 the solvent power of the decanted fluid and of normal goat serum. 

 The figures, I-V, correspond to the blood-cell amboceptor com- 

 bination employed in the previous tables. 



TABLE IV. 

 BRIEF ABSORPTION OP GOAT SERUM WITH RABBIT BLOOD. 



Complements I, II, and III have been completely preserved, 

 IV and V have been reduced to one-fourth and one-seventh respec- 

 tively, thus furnishing another proof for their diversity. It is of 

 special interest that in this brief action the particular activating 

 principle (complement II) which we shall term the " dominant com- 

 plement " has not at all combined with the cell, whereas other com- 

 plements, which are of no consequence so far as the solvent process 

 is concerned, have already been subjected to a distinct absorption. 



With the absorptions are also to be classed the experiments con- 

 cerning Case I, which we have made with guinea-pig blood stro- 

 mata obtained after the method of H. Sachs l by heating the blood 

 to 55 C. In these stromata the receptors which bind the ambo- 

 ceptors present in normal goat serum have been preserved capable 

 of reacting. 



These experiments demonstrated the absorption of the comple- 

 ments for the two normal hsemolysins (Cases I and II) while the 

 rest of the complements were in the main preserved. 2 An experi- 

 ment of this kind is shown in Table V. 



1 See page 167. 



2 In this also it is necessary first to determine the favorable conditions 

 governing the experiment. Thus, in order to completely bind the guinea-pig 



