THE H^MOLYTIC PROPERTIES OF ORGAN EXTRACTS. 281 

 TABLE X, 



The serum was diluted five times with tap-water and after heating the 

 corresponding amount of salt was added. 



This experiment shows that the goat serum, which in amounts of 

 0.2 cc. almost completely neutralizes three times the solvent dose of 

 the emulsion, does not suffer the slightest loss of action even when 

 heated to 100 C. for one hour; that an antibody in the proper sense 

 is, therefore, not present. 



Whether the coctostable substance which acts here is a simple 

 unit which acts specifically on the haemolytic substance of the organ 

 extract, or whether we are dealing with a complex of bodies having 

 an "antireactive" action, can only be determined by further investi- 

 gations. 1 



The haemolytic substances found in organ extracts and examined 

 by us are, therefore, 



1. Coctostable; 



2. Soluble in alcohol; 



3. Not complex; 



4. Not able to excite the production of antibodies. 



This shows that we are dealing with substances which are entirely 

 distinct from the haBmolysins of serum and which belong into a 

 peculiar class of substances acting haemolytically. 



1 Analogous actions of coctostable substances have recently been observed 

 by Korschun, who has described a "pseudo-antirennin" of normal sera 

 (Zeitschr. f. physiol. Chemie, Vol. 36, Nos.. 2 and 3, 1902). A thermostable 

 substance inhibiting the action of urease has also been recently described by- 

 Moll (Hofmeister's Beitrage, Vol. II, Xos. 7-9). 



