STUDY OF YUCCA ANGUSTIFOLIA 133 



tile principle (0.2 per cent.) in extract (2), and with traces of 

 chlorophyll in extract (3). 



Fixed oil (i) was crystalline in structure. It was soluble 

 in ether, chloroform, benzole, carbon di-sulphide, and amyl 

 alcohol; incompletely soluble in cold or boiling alcohol, acetic 

 ether, and ammonium hydrate. It was colored pale green 

 by sulphuric acid of 1.634 specific gravity, and changed to 

 a bright-green color by calcium di-sulphide, but formed no 

 emulsion with it. Phosphoric acid colored it yellow. The 

 fixed oil was saponified, and a white soap separated. This 

 was decomposed, and the fatty acids recovered. Glycerin 

 was separated from the soap filtrate. 



Fixed oil (2) was dissolved with difficulty in boiling 95 per 

 cent, alcohol, and hardened and discolored by absolute alcohol. 

 It was not saponified. Crystalline solids were separated by 

 precipitating the alcoholic solution with magnesium acetate. 

 They melted at 85 C. and at 60 C, respectively. 



Fixed oil (3) was soluble in alcohol, ether, chloroform, 

 benzole, carbon di-sulphide, oil of turpentine, almond oil, 

 glycerin, and slowly soluble in acetic ether. The presence of 

 free fatty acids was demonstrated. The fixed oil was colored 

 dark-green by syrupy antimony chloride; on adding to it sul- 

 phuric acid of 1.475 specific gravity, and a small quantity of 

 zinc, hydrogen was generated, and the solubility of the oil in 

 the acid liquid was accompanied by a rosy tint given to the 

 solution. 



Fixed oil (4) was crystalline in structure. It was soluble 

 in warm absolute alcohol, in cold acetic ether, chloroform, 

 benzole, amyl alcohol, ether, carbon di-sulphide, and glycerin. 

 It was saponified, and a white soap separated. The fixed oil 

 was colored dark-brown by alcoholic ammonia, and a mix- 

 ture of ferric chloride solution and powdered rosaniline gave 

 a violet-colored reaction with it. 



These fixed oils differed in their physical characters and 

 chemical reactions. This difference may be due to the pre- 

 sence of free fatty acids and glycerides in varying proportions 

 in the four parts of the plant. It is of interest to note that in 

 the subterranean part of the Yucca, the oil extracted from 



