164 PLANT AND ORGANIC CHEMISTRY 



cent, alcohol, dried, and weighed, deducting ash. It con- 

 sisted of mucilaginous substances and albuminoids. 



Weight of precipitate by 90 per cent, alcohol yielded 0.85 per cent. 



ash yielded 0.25 " 



Lassaigne's test showed the presence of albuminous sub- 

 stances. 



The filtrate and wash alcohol from the 90 per cent, alcohol 

 precipitate was evaporated to dryness, and weighed, deducting 

 the amount of soda acetate. It gave 0.24 per cent. The resi- 

 due soluble in water was mixed with acetate of copper solu- 

 tion. A very small quantity of albuminous substances was 

 precipitated by the reagent. 



The albuminoids of the bark were estimated from the total 

 nitrogen in one gram of the original powder. It yielded 4.75 

 per cent, of albuminoids. 



The powder insoluble in dilute soda solution was washed 

 with distilled water. The liquid was deeply colored. It was 

 evaporated, and the amount of solids estimated. It gave 1.3 

 per cent. 



Extract (2), the Wood of the Root. 



The powder insoluble in water was treated in the same way 

 as in extract (i). The filtered solution was mixed with 90 per 

 cent, alcohol in the manner described. The precipitate was 

 estimated, deducting ash. 



Weight of precipitate by 90 per cent, alcohol yielded 2.170 per cent. 



ash yielded 0.256 " 



The filtrate from the 90 per cent, alcohol precipitate was 

 treated with water, and the soluble matter precipitated by 

 copper acetate. The precipitate was collected, dried, weighed, 

 and ignited, the resulting oxide of copper being deducted. It 

 yielded 0.104 P er cent, of albuminoids. A current of washed 

 carbonic acid was passed through the dilute soda extract to 

 determine the presence of globulin (vitellin, myosin), and 

 with negative results. The albuminoids were determined from 





