DORYPHORA DECEMIJNEATA. 43 



sible to draw anaphases of the second division from a polar view and 

 the lateral view showed nothing unusual, merely the longitudinal 

 division of all of the chromosomes. 



The spermatids show some interesting variations from the other 

 species which have been examined. In figures 178 and 179 we have 

 telophases of the second spermatocyte, showing centrosome and archo- 

 plasm (fig. 178) and certain masses of deeply staining material in the 

 cytoplasm (fig. 179, a^). Figures 180 and 181 are young spermatids 

 showing the archoplasm from the second spindle ( 2 ) and a smaller, 

 more deeply staining mass (#0, derived from the irregular masses of 

 the earlier stage (fig. 179, a^. In figures 182 and 183, the axial fiber 

 has appeared and the larger mass of archoplasm (# 2 ) is being trans- 

 formed into a sheath. The other body remains unchanged. During 

 the following stages this smaller archoplasmic body (a^ lies in close 

 contact with the axial fiber and sheath (0 2 ), and gradually decreases 

 in size (figs. 184-186) until it disappears in a slightly later stage. 

 The acrosome seems to develop directly out of the cytoplasm. The 

 enigmatical body (0j), which is probably archoplasm from the first 

 maturation spindle, as it is not found in the cytoplasm of the first 

 spermatocyte, may serve as nutriment for the developing axial fiber. 

 The sperm head has a peculiar triangular form, staining more deeply 

 on two sides. 



Miscellaneous Coleoptera. 



Considerable material from the spruce borers was collected at 

 Harpswell, Maine, but the species were not identified. Although 

 these insects were in the pupa stage, most of the testes were too old. 

 There were no dividing spermatogonia and few spermatocyte mitoses. 

 Most of the spermatocytes contained 10 chromosomes, one of which 

 was plainly an unequal pair. In a few testes the number was u, 

 indicating that pupae of two species had been collected. Figure 187 

 shows the metaphase of first spermatocyte mitosis with the unequal 

 pair in metakinesis. Figures 188 and 189 are first spermatocyte 

 equatorial plates of the two species, containing 10 and n chromo- 

 somes respectively. Figure 190 is a first spermatocyte spindle in 

 anaphase, showing the unequal pair behind the other chromosomes. 

 Figure 191 is an equatorial plate from a second spermatocyte, showing 

 the small chromosome. In figure 192 are shown several of the biva- 

 lent chromosomes, including the unsymmetrical pair, from nuclear 

 prophases of the first division, all from the same cyst. 



Adalia bipunctata (family Coccinellidse), the common lady beetle, 

 has a very conspicuous pair of unequal heterochromosomes, as may be 



