38 



ELEMENTS OF WATER BACTERIOLOGY 



BACTERIAL CHANGES IN WATER DURING STORAGE 

 (WHIPPLE, 1901) 



1 0.0005 P er cen t peptone added to the 



water. 



WolfThiigel and Riedel (Wolffhiigel and Riedel, 

 1886) noted the dependence of this multiplication 

 on the air-supply, vessels closed with rubber stoppers 

 showing lower numbers than those plugged with cotton. 

 Similarly, Whipple found that the multiplication of 

 bacteria was much greater when bottles were only 

 half full than when they were filled completely; and also, 

 as shown in the very striking table on page 39, that 

 the size of the bottle markedly influenced the growth. 



An important series of investigations by Kohn (1906) 

 suggests that this phenomenon of multiplication dur- 

 ing storage may be due in part to the solution of certain 

 constituents of glass which favor bacterial life, since the 

 increase is notably greater in bottles of the more soluble 

 glasses. 



