iv MICKOBES SIMULATING THE B. PESTIS 59 



The post-mortem examination of the above rat showed 

 the following condition : — The inguinal glands were not 

 enlarged. The spleen was enlarged, dark, and very juicy. 

 Both lungs were very congested, showing in addition 

 patches of consolidation. In the spleen and in the in- 

 flamed parts of the lung a variety of microbes were found 

 in stained film specimens, and amongst them some which 

 owing to bipolar staining might have passed for B. pestis. 

 In the film specimens of the spleen in particular the great 

 majority of the microbes were shorter or longer oval bacilli 

 showing bipolar staining. 



Subcutaneous injection of a few drops of the inflamed 

 lung juice into a guinea-pig (No. 1) caused illness within 

 twenty -four hours, viz. the animal became quiet and 

 developed local tumour. It was found dead on the second 

 day. The post-mortem examination showed the following 

 condition : — The subcutaneous tissue of the groin, abdomen, 

 and chest of the injected side — the injection having been 

 made in the groin — was much infiltrated with sanguineous 

 fluid. In this fluid crowds of bacilli were present, of the 

 same size and staining as those mentioned above as present 

 in the lung juice of the rat. The inguinal glands on the 

 injected side were swollen and firm ; and in them were 

 much blood and numerous bacilli of the same character as 

 those already mentioned. Both lungs were hypersemic ; 

 sanguineous exudation was present in the pericardium ; 

 the spleen was not enlarged ; the suprarenals were deeply 

 congested. 



Cultures were made, not only from this animal, but also 

 from a large number of other animals, both guinea-pigs 

 and rats, inoculated in series from it ; so that the morpho- 

 logical, cultural, and physiological characters of the microbe 



