116 ORIENTAL PLAGUE chap. 



of an animal (of the same species) dead of acute plague. 

 Nevertheless in the experiments in question such material 

 proved barren of a high degree of virulence, the chief 

 manifestation of disease induced being a local bubo, with 

 delay of fatal result. Furthermore, this attenuated 

 B. pestis presented the morphological and cultural 

 characters (conspicuous shortness of the bacilli in culture, 

 transparency of growth on gelatine in the earlier phases) 

 of the type of B, pestis which I have characterised as 

 type 2. 



Series B 



A considerable number of experiments was made with 

 virulent B. pestis that had been obtained on January 31, 

 1901, from the bubo of a man dead of plague who had 

 worked in a plague-infected flour-mill in Cardiff. The 

 subculture of this strain (as also of a rat — strain from 

 the same flour-mill) is at present — end of 1903 and 

 beginning of 1904 — of normal high virulence : a trace of 

 a forty-eight hours old growth on agar inoculated 

 cutaneously into a rat causes (fatal within seventy-two 

 hours) acute septicemic plague of the typical character. 



Experiment 5. — With a trace of a forty-eight hours 

 old agar growth of B. pestis from the Cardiff bubo, a rat 

 was inoculated cutaneously on left side of its tail-root. 

 The animal was found dead in about sixty to sixty -six 

 hours. Post-mortem examination showed the following 

 condition : — Scab on site of inoculation ; left inguinal 

 glands much enlarged and deeply congested, in parts 

 purple, hemorrhagic ; spleen much enlarged, dark, firm ; 

 intestines congested, relaxed and full of sanguineous 

 mucus ; kidneys and liver much congested ; both lungs 



