120 ORIENTAL PLAGUE chap. 



spleen enlarged ; upper lobe of right lung deeply con- 

 gested. Inguinal glands and spleen contained abundance 

 of B. pestis of type No. 2. 



This, then, was a case of distinct septicsemic plague, 

 differing from the typical form (virulent type 1) only in 

 the fact that death was delayed, and that the colonies 

 derived from both the bubo and spleen were of the same 

 transparent variety as those of the B. pestis used for 

 inoculation. 



Experiment 9. — A further rat, 1m, was inoculated 

 cutaneously with a fair amount of the (transparent) 

 growth of a culture from the spleen of rat 1l. This 

 animal died on the fifth day. On post-mortem examina- 

 tion the inguinal glands of the inoculated side were 

 found slightly enlarged, the spleen very slightly enlarged. 

 Cultures of the spleen and heart's blood yielded no 

 growth. 



Experiment 10. — A further rat, In, was inoculated 

 cutaneously with the growth of a two days old agar 

 subculture (second remove) of the same stock (spleen of 

 rat 1l) as in the preceding experiment. This animal was 

 distinctly ill on the third and fourth days, being quiet, 

 not feeding, its coat slightly rough. The control rat 

 inoculated on same day as In with culture of the same 

 date (two days agar) of the Cardiff bubo type was found dead 

 in sixty to sixty-six hours with severe plague. But rat In 

 on the sixth day, though still ill, was alive. On the 

 fifteenth day it was still not feeding well and had a slightly 

 rough coat, but it was better in respect of moving about 

 freely. The animal was now killed and showed the 

 following appearances : no bubo ; spleen not enlarged ; 

 ileum congested ; Peyer's glands marked ; liver normal ; 



