188 OMENTAL PLAGUE chap. 



with typical subacute plague, bits of the necrotic bubo, 

 and pieces of the spleen and liver (full of minute nodules), 

 were finely minced and mixed separately in glass dishes 

 with earth (fine gravel) and fine sand. These materials 

 were then so placed as to obtain fairly free access of air, 1 

 and were left to dry spontaneously. 



On July 14, i.e. after one week, a small amount in 

 each case of the dry material was emulsified in warm 

 water, and of each emulsion about J to \ cc. was sub- 

 cutaneously injected into a guinea-pig. These guinea-pigs 

 will be designated : — 



No. 12. — "Sand-plague" guinea-pig. 



No. 13. — "Earth-plague" guinea-pig. 



Guinea-pig No. 12 was found dead on July 18, 

 i.e. after four days ; it had, however, been dead for some 

 time, July 17 being a Sunday. The post-mortem 

 examination showed typical plague (early stage of sub- 

 acute), viz. necrotic bubo, and few minute nodules 

 in spleen and liver. Bubo and spleen were crowded 

 with B. pestis, as shown by film specimens and by 

 cultures. 



Guinea-pig No. 13 was dying on July 21. On post- 

 mortem examination there was found a big necrotic bubo 

 crowded with B. pestis ; the spleen, liver, and the lungs 

 were crowded with necrotic [[nodules and -patches ; film 

 specimens and cultures yielded pure cultures of B. pestis J% 



Experiment 7. — The materials of experiment 6 were 

 used for inoculation of guinea-pigs after a further week of 

 drying, viz. on July 21. By this date the materials 

 appeared perfectly dry and hard as bricks. 



1 The dishes were kept under a bell glass, raised all round about £ to 1 inch 

 and placed by the open window. 



