218 



ORIENTAL PLAGUE 



CHAP. 



It is seen from this series that the triple intraperitoneal 

 injection of sterile culture was less effective as to the 

 production of agglutinins than had been subcutaneous 

 injection of like material ; that the subsequent two intra- 

 peritoneal injections of small doses of living culture 

 brought about distinct presence in the blood of agglutinins ; 

 and that while further similar injections continued to 

 enhance the agglutinating power of the blood, the ninth 

 injection was followed by a distinct decrease of this 

 power. 



3. Guinea-pigs Nos. 5 and 6. — These two animals 

 (half-grown) were repeatedly injected subcutaneously with 

 Hajfkine prophylactic. 



The animals were injected on the following dates, and 

 with the following amounts : — 



No. 



Date of 

 Injection. 



Method of 

 Injection. 



Material (and Amount) Injected. 



let 



2nd 



3rd 



4th 



5 th 



6th 



7th 



June 27 

 July 3 



„ 18 . 

 August 1 



„ 29 . 

 September 17 

 October 21 . 



Subcutaneous 



10 cc. of Haffkine's prophylactic. 



o 

 10 

 10 

 10 

 10 

 10 



The blood serum was tested (dilution 1 : 20) on the 

 following dates, and with the following results : — 





