IX 



PROTECTIVE INOCULATION 



255 



culture following on a third intravenous injection of sterile culture 

 causes, on the second day, a very considerable rise of the body 

 temperature, lasting for more than a week. It is curious, however, 

 to find that the animal did not lose in weight till the temperature 

 again commenced to fall. But, as mentioned above, the body weight 

 is of very uncertain value. It has to be added in this connection 

 that the animals were always kept as to food and all other conditions 

 under precisely the same favourable conditions. 



Babbit No. 3 



First subcutaneous injection with 10 cc. Haffkine prophylactic on 

 June 17 : — 



As has been recorded in connection with various experiments 

 here reported on, this animal was injected and reinjected sub- 

 cutaneously a second, third, fourth, fifth, and sixth time, on each 

 occasion with 10 cc, and a seventh and eighth time, on each occasion 

 with 20 cc. of Haffkine's prophylactic. But there was no corre- 

 sponding alteration observed in the body temperature. The body 

 weight, however, was constantly varying upwards and downwards in 

 an erratic fashion. It is, however, seen that the first injection 

 caused a sudden and considerable rise of temperature, lasting for 

 two or three days — that is to say, a change of the same kind as was 

 noted in the case of rabbit No. 1 after the first intravenous injection 

 of that animal with sterilised bacteria taken from solid medium. 

 Subcutaneous injection, therefore, into a rabbit of under 1200 grammes 



