x MODES OF DESTRUCTION OF B. PESTIS 297 



Cultures were made on gelatine (21° C.) and agar 

 (37° C), and inspected on April 16, with the following 

 result : — 



1. — Phenol 1 in 80, 5 minutes' exposure. On gelatine 3 doz. colonies. 



On agar „ 



10 „ „ In both tubes no growth. 



1 ^ 



xu 35 53 35 5) JJ 



2. — Formalin 1 in 30, 5 minutes' exposure. Crowds of colonies in both 



tubes. 

 10 „ „ Numerous colonies. 



15 „ „ About 5 dozen colonies. 



3. — Cyllin 1 in 2000, 5, 10, and 15 minutes' exposure. All tubes free 



of growth. 

 1 in 1800 



1 in 1600 



33 



35 



Series V.— April 11, 1904. 



A. — Watery distribution of cyllin . . 1 in 2400 

 B.— „ „ . 1 in 2200 



Time of exposure, 5, 10, and 15 minutes. 

 Cultures were made on agar (37° C.) and gelatine 

 (21° C), and inspected on April 18, with this result : — 



1. — Cyllin 1 in 2400, 5 minutes' exposure. Gelatine tube had one 



colony of JB. pestis. 

 The agar tube had a 

 small amount of 

 growth in the con- 

 densation water ; this 

 was allowed to spread 

 over the sloped surface 

 of the agar, with the 

 result that copious 

 growth of B. pestis 

 covered the surface 

 next day. 



10 „ „ No growth in any tube. 



-*■ t) <• «« jj jj jj 



