[ 59 ] 



At 4.30 they were found to have separated. They were left together 

 overnight. 



On the morning of the 7th, this female F 1 was removed to another 

 vessel beside a male, M 5, hatched overnight. He flew, at her instantly 

 on her arrival and in a few seconds mating was effected, 9.55 a.m. 

 At 11.20 a,m. they were found to have separated. Mating (Mb -\- F\)\. 



F 1 was now placed in a small glass vessel over some moist cotton 

 wool. Up till 6.35 oviposition had not taken place under these 

 conditions. At this hour she was placed over soil to see if this would 

 now supply the stimulus. The result was negative. This female, 

 however, was known to have oviposited previously, and at this stage 

 she had lost the full-bodied appearance of the unmated female and was 

 quite slender in form. 



On the 9th, F 1 was placed with another male, M 6, but no union 

 took place. From this date she was kept in the company of a male 

 until she died. This occurred upon the 16th and meantime no further 

 mating had been effected in her case. Reviewing the facts regarding 

 her, it is noted that between the morning of the 5th and the morning 

 of the 7th she mated at least six times, and with three different males. 

 She lived 11 days in captivity. 



The male M 1, as has already been recorded in the account of the 

 behaviour of F 1, mated with her four times in the course of two days. 

 He also mated with another, F 2, within a short time of her hatching. 

 This took place at 7.10 p.m. on the 6th July. At 9.15 they were 

 still mated, and on the following morning they were again found in 

 coitu. 



At 10.35 upon the morning of the 6th this male was introduced 

 to § vessel in which there were already three females and one male. 

 He at once flew amongst these and in a few seconds became coupled 

 to a hitherto unmated female F 3. They remained in coitu until 12.15 

 when they separated. Subsequently as recorded for' the female, F 1, 

 he paired with her at 2.40 p.m. of the same day. 



No further opportunities for mating were given to this male until 

 the 9th (four days after he was first found mated), on which day he 

 was placed with a newly hatched female F9 at 11 a.m. They were 

 left together until 3.20 p.m. but coitus did not take place. He mated 

 at least seven times in the course of two days. He died upon the 12th, 

 having lived seven days. 



A number of similar experiments were performed and always with 

 consistent results. Recently hatched insects paired most readily, and 



