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were found in front of tlie swarm, but a large number was seen in front of tbe 

 parent stock. Both hives were examined comb by comb on this occasion, 

 and found to have queens and brood in all stages. The swarm was small in 

 number, but lively, and apparently quite healthy. The parent stock was 

 more numerous, but a considerable number of bees showed dislocation of the 

 wings, an indication which, however, has little importance as a symptom of 

 the disease. On the 12th September, sample bees were taken from the 

 parent stock. These were picked off the combs, bees with dislocated wings 

 being specially selected. Owing to unlavourable weather conditions there 

 were no bees out of doors at this date. Microscopic examination showed no 

 recognisable traces of Nosema. 



The hives in which the bees were housed had no previous history of Isle 

 of Wight disease. The bees were too few to keep warm in the hives during 

 winter, and, on this account alone, survival was not possible. 



Eighth Attack. One Stock, Fl^,. — Without any disinfecting measures. 

 Hive No. 14, which housed the previously mentioned infected stock (E14), 

 Was used for a lot of Welsh driven bees procured in the late autumn of 1913. 

 The infected combs were also retained with contained honey. In early 

 spring, 1914, heaps of dead were found on the floor ; later, on fine days many 

 " crawlers " were observed. At the beginning of April, the bees covered only 

 a single comb, but they were carrying pollen freely, and there was a patch of 

 brood. Crawling had now ceased, the stock built up during the summer of 

 1914, wintered on natural stores, and was quite strong in the spring of 1915. 

 On l7th June 1915, J. E. examined six live bees, active after journey (posted 

 14th), all parts normal, no Nosema observed. Other samples of bees which 

 were sent to Aberdeen for examination arrived dead, and were not satisfactory 

 for Isle of Wight disease diagnosis. On the other hand, it can be stated 

 positively that Nosema spores were not present in the bees examined. Bees 

 were examined individually, and also pulped in groups; The colons con- 

 tained masses of pollen, but Nosema was not found. A stained smear was 

 also made of one bee, and this gave a negative result. 



In August 1915, the stock was found weak and queenless. In October it 

 was quite extinct, and the local observer (Mr Gibson) reported that crawling 

 had been very marked. 



In October the hive was restocked with a new lot of driven bees from Wales, 

 the old combs and stores being retained. Efforts were made to put this stock 

 in good condition for wintering, but the weather was rather cold while 

 feeding was going on. 



This is now the third stock housed in the same hive in which two previous 



