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queenless, we re-united the two with the original queen on the 23rd 

 of July, making a stock of over seventeen frames of bees. 



During summer the stock continued in good strength, and no 

 sign of disease was ever observed. In September one frame of 

 bees was removed for experimental purpose, and on the 27th a 

 clearing board was introduced and the surplus honey removed, and 

 the bees were made up for winter in the lower box. Two pounds 

 of Bacterol candy were left on the frames. On various occasions 

 throughout the experimental period bees were examined in 

 numbers. Nosema apis was not found, and no signs of Isle of 

 Wight disease were ever observed. 



No. 4. On the 17th August 1918, to a stock of bees^ covering about 

 six frames and headed by a young queen (Italian bred, mated in 

 Aberdeen) of the current year we added a lot of bees together with 

 brood and stores upon four frames, the remnant of a stock reduced 

 to this condition by Isle of Wight disease. Its previous history 

 is as follows : — One week after swarming, viz. 7th July, indications 

 of Isle of Wight disease were observed, and during that month the 

 stock was repeatedly treated with flavine, notwithstanding which 

 the bees continued to crawl until the stock was reduced to the 

 condition stated upon the date mentioned. The union of the two 

 lots was effected without loss of bees, and the condition of the 

 increased stock was ascertained upon 29th August to be as 

 follows : — The bees covered ten frames fully, and there was brood 

 upon six, including eggs. The queen was seen upon the frames, 

 and there were no signs of disease outside the hive. Stores were 

 short. The weather was changeable, cold to mild. 



During the following month the weather was mostly wet, and 

 there were few indications of disease outside the hive. On 20th 

 September it was ascertained that there was brood upon one frame 

 only, and the bees were covering nine frames. No crawling amongst 

 the Italian bees was observed throughout the autumn, and the bees 

 were put up for winter. The mortality up till the end of February 

 was slight. The bees were flying at this date in bright but cold 

 weather. This stock has wintered successfully, and in fine weather 

 the bees have been seen gathering pollen. 



Winter Experiments. — No. 5. In the autumn of 1916 a 

 stock of bees which had been showing the usual Isle of Wight 

 disease symptoms intermittently over a considerable period was 

 treated with Bactero! both by feeding and spraying. This had no 

 permanent effect. 



At the beginning of October crawling was much in evidence. 

 There was sealed brood upon two frames, but the stock was greatly 

 diminished in numbers. In the endeavour to maintain the stock 

 throughout the winter, we added a lot of driven bees obtained in 

 Wales at the beginning of November. The united stock was 

 ascertained a week afterwards to have clustered over six frames. 

 On fine days the bees were flying, and signs of the disease were in 

 abeyance. At the end of December on a fine day bees from this 

 stock were flying and removal of dead was observed. Again on 



' These bees had an earlier history oi Nosema infection and of contact with 

 brood of Isle of Wight diseased stock. 



