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to this date was not again observed. The stock, which was short 

 of stores, was fed with Pascall candy and put up for winter. 



On 23rd January it was found to be much reduced in numbers, 

 but to have plenty stores. It was still in existence on the 5th 

 February, but on the lOth it had died out. The queen was found 

 apparently dead also, but on removal and warming she revived.^ 



On more than one occasion bees were removed from this stock 

 for experimental purposes in the late autumn. The cause of its 

 dying out is not clear. Our impression on reviewing the whole 

 history was that the new stock built up by the Welsh queen intro- 

 duced to unhealthy bees never contracted the disease. 



In one of the apiaries, Isle of Wight disease developed generally 

 during the working season, 1917. A number of the stocks were 

 re-queened. The season proved highly successful for honey, 

 although the disease continued. The stocks were wintered, but 

 before spring all were dead. 



Season 1918 in the same apiary. On August 23rd, a sick 

 stock was re-queened with a young imported Italian queen. Six 

 weeks afterwards there were Italian bees, offspring of this queen, 

 amongst the crawling diseased bees. A duplicate experiment 

 also yielded the same result, offspring of this second Italian queen 

 being found amongst the crawling bees at the end of six weeks. 



Our conclusions regarding re-queening are that, whilst it may 

 be useful temporarily in the early part of the working season, it is 

 not successful in ridding a stock of the disease. 



Experiments with Brood of Diseased Stock — With a view to 

 obtaining information as to the possible infectivity of the brood 

 in relation to this disease a number of experiments have been 

 performed, of which the following are typical examples. 



No. I. This stock was obtained in the spring of 1918. Samples 

 of the bees were microscopically examined on several occasions. 

 At the end of May two bees were found heavily infected with 

 Nosema apis, and again this parasite was found in two bees at the 

 end of June. These occurrences were exceptional, as in numerous 

 other instances such examinations yielded negative results, and the 

 parasite was not found after the latter date specified above. It is 

 certain there was no general infection with this parasite. 



The stock about the middle of May was covering six frames and 

 had young brood and eggs upon five, besides sealed brood. 

 Increase was rapid, and before the i8th of June the stock had yielded 

 four frames of bees for the formation of nuclei or strengthening of 

 other stocks. 



On the loth June one frame of brood from each of two Isle of 

 Wight disea.sed stocks was introduced. Again on the 18th a third 

 frame of brood, also from a similarly diseased stock, was introduced. 

 This brood had all hatched by 6th July, and the frames were in 

 ordinary routine utilised by the queen in egg-laying. The stock 

 steadily built up, and by the middle of June was thickly covering 



' The queen of this stock was subsequently given to the remainder of an Isle 

 of Wight diseased stock, and later to one frame experiment of date February 

 28th, 1918. She later headed a Nosema experiment in May. 



