13' 



twenty frames. There was a large quantity of surplus honey, while 

 the second brood box often frames also contained considerable stores. 



No signs of Isle of Wight disease have ever been observed in 

 this stock, which on ist November remained strong in numbers and 

 healthy. During the whole season it has stood in an experimental 

 apiary into which Isle of Wight diseased stocks have been intro- 

 duced as a matter of routine throughout the summer and autumn. 

 Further, it actively robbed several of these stocks early in August. 

 The presence of Nosema apis amongst the bees has already been 

 recorded. 



From the foregoing it may be gathered that the whole of the 

 bees introduced as brood or eggs from the diseased stock was 

 hatched by the 9th of July. Since no signs of disease appeared 

 amongst the bees from that date onward until the bees wintered, it 

 appears that the brood was healthy although bred and a good 

 proportion of it (sealed brood) was nurtured in the affected hive. 



No. 2. On isth May 191 8 an Italian queen was introduced to 

 a two frame nucleus of queenless black bees. Nosema apis had 

 been found in the attendant bees which accompanied this queen 

 from Italy. On the 24th the queen was seen upon the frames, 

 and there were larvae and eggs in the brood nest. 



On the 27th May one frame with brood in all stages, but without 

 bees taken from a stock suffering from Isle of Wight disease, was 

 introduced. Another similar frame was given upon the 31st, and 

 another upon the nth June. Upon the istof July three additional 

 frames containing brood in all stages taken from another Isle of 

 Wight diseased stock were placed in the hive, making six frames in 

 all. The patches of brood in all cases were of moderate size. The 

 introduced brood duly hatched, and the nucleus continued to prosper 

 in a normal manner. On the 1st August the stock was making up 

 well, the bees were covering nine frames and the brood nest 

 extended to seven. This represents the maximum strength so far 

 as was observed. 



It will be noted that all the brood introduced from the diseased 

 stocks had hatched out by 22nd July. These bees were recognizable 

 by their difference in colour from those bred by the queen. During 

 August they represented a fair proportion of the bees present. 



During the whole course of the experiment the stock was 

 watched for indications of the presence of disease, and nothing in 

 the appearance or behaviour of the bees, either black or yellow, 

 suggestive of the disease was ever seen. The stock has wintered 

 successfully. 



No. 3. On 27th May 1918 a stock of Punic bees covering five 

 frames which had wintered in Aberdeen was given a frame of brood 

 from an Isle of Wight diseased stock. Again on 31st another 

 frame was given from the same source. On nth June a third 

 frame of similar brood from another diseased stock was added. The 

 stock increased in numbers rapidly to the middle of July, when the 

 queen was found to be missing and many queen cells were being 

 made. These were removed and a queen introduced, but on 20th 

 July the stock swarmed, and on ist August the parent stock was 

 covering twenty frames and storing well, but no surplus has been 



