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obtained. The last bee from the Isle of Wight diseased stock was 

 due to hatch on 2nd July. Such bees were present in the stock 

 from 27th May onward. On no occasion have any indications of 

 Isle of Wight disease been observed in the stock, which has 

 wintered successfully. 



No. 4. On 4th July two frames of brood from a stock suffering 

 from Isle of Wight disease were introduced to a small swarm of 

 Irish bees covering five frames. The stock continued to work 

 normally during the summer and at the beginning of August was 

 covering nine frames with brood upon eight. There were plenty 

 stores. The whole of the brood introduced from the diseased 

 stock was due to be hatched upon the 2Sth July. There have been 

 no signs of Isle of Wight disease in any of the bees, and the stock 

 has wintered successfully. 



No. 5. On 4th July a swarm of bees from Tipperary covering five 

 frames was given two frames of brood from a stock suffering from 

 Isle of Wight disease. The stock built up well during the summer, 

 and in the middle of August had a good supply of stores. There 

 were ten frames of bees, nine of which were carrying brood. The 

 last bee from the Isle of Wight brood was due to hatch on 2Sth 

 July. No indications of disease have ever been observed in this 

 stock, which wintered successfully and has become the subject of 

 another experiment. 



No. 6. On 14th July 19 18 twenty frames of brood belonging to 

 an Isle of Wight diseased stock, originally of thirty frames, were 

 placed with a healthy stock, very strong in bees. This stock was 

 isolated in a heather area at the beginning of August. It did well, 

 and is now wintering in good condition, having yielded a large 

 surplus. No signs of disease have ever been seen. 



In the selection of the brood in the foregoing experiments the 

 endeavour was always made to utilise the maximum amount of 

 sealed brood. In this way bees were taken which had had the full 

 opportunity for receiving infection from attendant bees. 



The results have been uniform throughout, and it would appear 

 that bees reared in a stock suffering from Isle of Wight disease are 

 not susceptible to the disease in the brood stage. Further, we 

 may add, dead brood has never been found to be a feature of the 

 disease. 



Incidental to these experiments we have found that whilst as 

 recorded the brood removed to a healthy stock did not develop the 

 disease, that which was left with the sick parent stock in due 

 course succumbed to it as adult bees. 



Experiments with Queens of Diseased Stocks. — With a view 

 to ascertaining whether bees associated with a queen from an Isle 

 of Wight diseased stock or bred from her in healthy surroundings 

 develop the disease, the following type of experiment was carried 

 out. 



Experiment No. i. — On the nth April 1917 we introduced to a 

 small stock of queenless Welsh bees, covering two frames, which 

 had been wintered in Aberdeen, a queen which survived from a 

 stock recently defunct and exhibiting Isle of Wight disease 



