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symptoms. Up to the middle of May progress appeared to be slow, 

 and the queen did not lay well. We removed this queen, and 

 replaced her by another queen surviving from a badly affected Isle 

 of Wight stock. During the following months the stock progressed 

 normally. No signs of disease could be observed, and at the 23rd 

 July the bees were covering ten frames with brood on eight. 

 August was very unfavourable to the bees generally. No surplus 

 stores were obtained, and feeding had to be resorted to before 

 winter. On the 24th January 19 18 the stock was found to be alive 

 and was supplied with Bacterol candy. On the 9th February the 

 bees were on six frames with brood sealed and unsealed on one. 

 At the end of February this stock was disturbed by workmen, and 

 losses appear to have been incurred through the bees flying out in 

 too cold weather. In the middle of March the hive was found 

 empt}' save for a few dead bees, including the queen. There were 

 very good indications that this stock was about to continue 

 satisfactorily in the forthcoming spring, and the failure appeared 

 undoubtedly due to the external cause indicated. We regard this 

 experiment as successful in showing that a queen surviving from 

 an Isle of Wight diseased stock may continue for a satisfactory 

 period (10 months) without communicating the disease. 



Experiment No. 2. — This stock consisted of bees obtained from 

 Wales in the autumn of 1916. It was wintered upon frames from 

 an Isle of Wight stock, and in February the bees were fed upon 

 Bacterol medicated candy, and meantime the Isle of Wight stock 

 frames were removed and replaced by others. At the end of March 

 the strength was low, amounting to about two frames. On the 28th 

 of this month the Welsh queen was removed and a queen from an 

 Isle of Wight stock was introduced. During April and May the 

 stock did moderately well, and at the end of May covered about 

 five frames ; the bees worked well, and at no time were there any 

 signs to indicate that Isle of Wight disease was developing. In the 

 beginning of May the bees robbed stores from an empty hive 

 containing the frames of a stock defunct from Isle of Wight 

 disease. The hive was closed, but the bees were making use of the 

 escape funnel at the top. 



Throughout the summer matters progressed well on the whole, 

 and on the loth July the stock consisted of ten frames good with 

 brood on nine. There was also a considerable amount of stores, 

 the brood nest being rather restricted. A super was put on on the 

 23rd, but the bees did not make use of it. The month of August 

 was extremely wet, and the bees had few opportunities for flight. 

 At the beginning of September the stock was still a crowded one ; 

 there was brood on eight frames, but the stores were running short. 

 Disease was constantly watched for, but no indications were ever 

 observed. The stock continued well and was fed with Bacterol 

 candy with a view to wintering. At the beginning of November it 

 was concluded that the queen, having been with the stock for over 

 seven months and no disease resulting, had failed to infect the 

 colony, and the experiment was regarded as concluded. At this 

 date the stock was divided and the bees utilised in other 

 experiments. 



