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Experiment No. 3. — This stock commenced with a nucleus of 

 one frame of bees, to which was given a queen from an Isle of 

 Wight diseased stock on 4th June. This queen headed the stock 

 up to 13th July, at which time it was strengthened with bees from 

 other sources, and at this date covered eight frames. At the time 

 of one of these additions the queen was lost. She was replaced by 

 another of similar history. This latter queen remained with the 

 stock, which at the beginning of August had a strength of nine 

 frames with brood upon seven, and was working very well. 



On 1 2th September stock No. 22, a similar experiment, was 

 united without its queen to this stock. The combined colony, 

 originally covering about thirteen frames, is now in one brood box, 

 with dummy excluder between. On 23rd the queen was seen upon 

 the frames. There zvere crawling bees on the plot in front of this 

 stock at this date, and subsequently the same feature was observed 

 on several occasions. 



The combined stock was found in January to have died out. 



Experiment No. 4. — On 15th March 1918 a queen surviving 

 from a stock depleted with Isle of Wight disease was placed upon 

 a frame with small cluster of healthy bees. The bees were kept in 

 an observation hive and fed with pure candy. Water was given 

 from time to time. They were also given candy mixed with 

 crocus pollen, and on other occasions with candy made up with pea 

 floUr. The hive was situated in the heart of the town and 

 opportunities for obtaining stores were limited. At the end of two 

 months there was a small cluster of young bees on the frames and 

 brood on the combs. 



This nucleus survived and in fact increased during May and 

 earlier part of June. 



On 1 2th June this lot was removed to another apiary and 

 strengthened with one frame of Punic bees. 



On loth July, having maintained itself normal, this nucleus was 

 added to another which had been headed by a queen of similar 

 Isle of Wight disease history from ist June, but which was at this 

 date found to have lost its queen. The strength of the united 

 stocks was six frames at this date, and from then onward until the 

 end of August the increase amounted to little more than one 

 additional frame. The queen was not a young one, which accounts 

 in part for the failure of the stock to increase, but at no time has 

 any evidence been obtained that Isle of Wight disease had 

 developed even in a minor degree. The nucleus was headed by a 

 queen surviving from an affected stock for a period of six months. 

 On 1 2th September this stock was united to No. 21, a similar 

 experiment. At the time of union the queen was not observed, 

 and subsequent search has shown that she is lost. There were 

 losses owing to the union, but these were small compared with the 

 total number of bees, and the joint stock was wintered up. As 

 recorded under experiment No. 3, disease developed here, and the 

 stock was found to have died out during the winter. 



Experiment No. 5. — On 15th July 1918 we removed the queen 

 of a strong stock of Punic bees with brood upon nine frames and 

 substituted a queen taken from a stock suffering from Isle of Wight 



