22 QUATERCENTENARY STUDIES IN PATHOLOGY 



the front of the abdomen possessing more of it than that from the leg. 

 In the former situation, also, the rods were elongating, and seemed to be 

 commencing to spore. There was about i oz. of peritoneal liquid, clear 

 and limpid. 



On June 6th, 1903, at 9 p.m., i c.c. of the liquid from the front of the 

 abdomen was mixed with 8 c.c. sterile distilled water, and 5 c.c. of the 

 mixture were injected subcutaneously into the left thigh of a Cheviot 

 hogg from ''clean" land, side by side with 5 c.c. of a i to 10 solution of 

 glacial acetic acid. 



June 7th, 8 a.m. — The animal was found to be lame in the inoculated 

 leg, and had a somewhat collapsed appearance. By 10 a.m. it was much 

 worse, and was leaning against the wall of the pen in a very depressed 

 condition. Seen again at 11*30 a.m., it occupied exactly the same 

 position, never having moved apparently since the last observation. At 

 9 p.m. the animal was prostrate and evidently dying. It was found dead 

 on the following morning. 



The appearances of the carcase were identical with those met with 

 in similar experiments, only the cedematous effusion was very much 

 greater than usual. It prevailed not only in the inoculated limb, ran 

 out of it when the skin was incised, but also spread upwards all over the 

 left side of the abdomen. The liquid was blood-stained and turbid, and 

 contained the bacillus in great quantity. Some of the rods were sporing, 

 others dividing. The cedematous subcutaneous tissue also showed some 

 haemorrhages and was blood-stained. 



Briefly summarized, this case is to the effect that a four-year-old ewe 

 was admitted to our Station af Kielder alive and suffering from Louping- 

 ill. The carcase did not present any visible morbid feature, but the inner 

 aspect of the intestinal wall, the mucous surface, was literally paved with 

 the Louping-ill bacillus. This, cultivated anaerobically, grew with 

 vigour, the growth consisting of Louping-ill rods, and when injected 

 subcutaneously into a fresh sheep, caused great local reaction, oedema, 

 and lameness of the inoculated limb. The animal was killed three days 

 after being inoculated, and some of the cedematous liquid from the front 

 of the abdomen was inoculated into a second sheep, killing it within 

 something like twenty-four hours, the cedematous liquid again permeated 

 with the specific rod. 



These are samples of observations I have verified over and over again. 



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