30 QUATERCENTENARY STUDIES IN PATHOLOGY 



There was not the slightest appearance of local reaction at the points 

 of injection. 



On the surface of the heart there were some patches of punctiform 

 extravasation, and the blood within the chambers was softly and only 

 partially coagulated. 



The brain and spinal cord were free from visible evidence of disease; 

 there was no meningitis. 



The peritoneal liquid, on being examined microscopically, appeared 

 to be quite free from germ impurity, the turbidity being caused by a 

 granular precipitate. 



Remarks. — This experiment was followed by very extraordinary 

 results, and is outstanding as the only one which we have made in the 

 inoculation of Louping-ill products where the symptoms assumed this 

 distinctly tetanic character. 



The Louping-ill bacillus was grown on 300 c.c. of glucose-beef- tea ; it 

 grew luxuriantly , the sediment of bacillus, which was copious, was 

 mixed with sheep's blood, covered with olive oil, and incubated ; the 

 greater part of the bacillary deposit was bacteriolysed ; the blood was 

 filtered and the serum injected subcutaneously into the sheep. Symptoms 

 did not show themselves until the twelfth day after the operation ; they 

 were ushered in with dulness, were followed by convulsive spasms of the 

 muscles, and terminated in those of ordinary tetanus, in which last con- 

 dition the animal died. 



It might be argued, of course, that the case was simply one of 

 tetanus, but against this theory is to be reckoned the fact that there was 

 neither wound nor external injury by which the animal could have 

 become infected, and the inoculation punctures had vanished without 

 leaving any sign of local reaction. 



Then again the sheep is not an animal which is liable to tetanus as 

 we ordinarily understand the disease. Reference has been made, how- 

 ever, to the tetanus-like symptoms which occasionally develop in the 

 course of Louping-ill, and these are sometimes accompanied by lock-jaw. 

 The accounts we have had from shepherds and others of such anomalous 

 cases, point to the animal becoming rigid while the jaw is firmly 

 clenched. Such cases evidently represent the disease under conditions 

 of the greatest severity. 



The long period of incubation might be adduced as against the 



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