STUDY OF THE IMMUNITY AGAINST BACILLUS PYOCYANEUS 9 



were covered with a thick layer of toluol, and were then repeatedly 

 shaken until complete sterility was obtained. 



Agar cultures were grown en masse in Roux's bottles, the bacterial 

 growth being subsequently washed off in a small quantity of normal 

 saline solution. The thick emulsion obtained in this way was then 

 removed by a pipette to a sterile stoppered bottle, and killed by toluol 

 or chloroform. 



Cultures in broth or on agar were also killed by heat at 65° C. and at 

 100° C, the toxicity being afterwards tested. 



Effect of Toluolised Bouillon Cultures. 



From these results it is manifest that Wassermann's so called 

 "toxin" can produce, in doses of '5 - I'O c.c. acute lethal effects. Post- 

 mortem, the main changes are witnessed in the peritoneum, which 

 presents evidences of acute inflammation, and in the liver and kidney, 

 which are inflamed, and show acute cloudy swelling. 



(165) 



