16 



quatercentenarv studies in pathology 



Effect of Broth Cultures killed at dj^ C. 



Effect of Bouillon Cultures exposed at 100° C for | hour 



Effect of Emulsions from 24. hour agar cultures killed by Toluol. 



(A 24 hour culture, which covered the whole surface of a Roux's bottle, was 

 emulsionised in 20 c.c. saline solution — the emulsion being then sterilised with 

 toluol.) 



Effect of 2^ hour agar cultures killed by chloroform. 



A 24 hour culture which covered the whole surface of a Roux's bottle 

 was exposed to chloroform vapour, the bottle being well closed by 

 an indiarubber bung. 



Before the action of the chloroform, o*i of a loopful of the living 



(166) 



