14 QUATERCENTENARY STUDIES IN PATHOLOGY 



In the 4th set (above window) growths were observed in the Anthrax 

 and B. typhosus tubes, while the Staphylococcus and B. coli remained 

 sterile. 



In the 5th set (floor of closet) growths were obtained in all the four 



tubes. 



Experiment 2. 



The second experiment was carried out in a small ward of the 

 City Hospital, Aberdeen. It had 3 close-fitting two-sashed windows 

 and an open fireplace, the chimney of which was loosely packed with 

 sheets of newspapers. The temperature of the room was 54° F. 

 The capacity of the room was 2,890 cubic feet. It may be noted that 

 in this experiment the proportion of formaline was doubled, 5 pints 

 of formaline and 16J ounces of potassium permanganate being used. 



Technique. — This was in all respects similar to that of the last 

 experiment, he micro-organisms used being : — 



(i) Sporing Anthrax; (2) Staphylococcus pyogenes aureus, (3) B. 

 coli, and (4) B. typhosus. Three sets of these organisms were made 

 for exposure to the action of the disinfectant, and were distributed 

 as follows, viz. : — 



1st set, on floor of room behind door. 



2nd set, on mantelshelf above fireplace. 



3rd set, on sash of one of the windows. 



They were exposed to the action of the disinfectant for 3 hours. 

 The room, on being opened at the end of the time, had a fairly powerful 

 odour of formaldehyde gas, although not quite so marked as in 

 experiment i. The different slips were then placed direct into bouillon 

 tubes, and incubated for 7 days at 37° C. Control slips, made at 

 the same time as those used in the experiment, were likewise placed 

 in bouillon tubes and incubated. Within 24 hours, a copious growth was 

 present in all the control tubes. At the end of 7 days, the following 

 results were observed in the tubes containing the exposed slips : — 



In the 1st set (floor of room) growths were observed in the Anthrax 

 and Staphylococcus tubes, while the B. coli and B. typhosus tubes 

 remained sterile. 



In the 2nd set (mantelshelf over fireplace), growth was present in the 

 Anthrax tube, while the Staphylococcus, B. coli, and B. typhosus tubes 

 remained sterile. 



(250) 



