4 QUATERCENTENARY STUDIES IN PATHOLOGY 



Guinea-pig 1 : — examined 27/7/05. 



Naked-eye appearances — 



No enlarged glands ; liver studded with fine nodules ; spleen 

 enlarged and congested — no nodules seen. Small intestines markedly 

 congested. Kidneys show minute punctate haemorrhages under the 

 capsule. Lungs somewhat congested. 

 Microscopical examination — 



Spleen smear shows bipolar organisms resembling B. pseudo- 

 tuberculosis ; many of these are in the cells. Heart-blood contains 

 fairly numerous bipolar organisms, some being in the mononuclear cells. 

 Animal experiment — 



The spleen and heart-blood were rubbed into a shaved area of skin 

 of a guinea-pig which died of haemorrhage from the lungs (probably 

 traumatic) within 24 hours. No organisms were seen in the heart-blood 

 of this animal, but a few bipolar organisms were observed in a spleen 

 smear. There was a cutaneous reaction at the site of infection, and the 

 inguinal glands were enlarged. 

 Cultural test — 



An organism was isolated from the heart-blood which corresponded 

 culturally, morphologically, and in certain sugar reactions with B. 

 enteritidis (Gaertner). 



Guinea-pig 2: — examined 31/8/05. 



Naked-eye appearances — 



Subcutaneous oedema ; no enlarged glands ; spleen very dark in 

 colour — markedly enlarged and studded with grey nodules exactly 

 resembling those of pseudo-tubercle. Very fine nodules were present in 

 the liver. Small intestines extremely congested. A small amount of 

 pleural and peritoneal effusion ; lungs apparently normal. 

 Microscopical examination — 



Heart-blood — very few bipolar organisms. 



Spleen and Omentum — very numerous bipolar organisms. 

 Cultural test — 



An organism similar to that from guinea-pig i was isolated from the 

 heart-blood. 



(290) 



