ON THE ACTION OF CERTAIN BACTERIA 5 



Guinea-pig 3: — examined S/O/^S- 



Naked-eye appearances — 



Spleen enlarged and studded with grey nodules. Mesenteric glands 

 enlarged and congested. Haemorrhages in the large intestine. Suprarenals 

 were congested and showed small haemorrhages. 

 Microscopical examination — 



Heart-blood — no organisms seen. 



Spleen — organisms, very like those of pseudo-tubercle, fairly numerous. 



Culture from heart-blood gave similar colonies to those previously 

 obtained. 



Guinea-pig 4: — examined 6/9/05. 



Naked-eye appearances — 



Spleen much enlarged, with yellow and grey nodules — a few also 

 in the liver. Small intestines congested. Lungs very congested. 

 Microscopical examination — 



Spleen — few, if any, organisms seen. 



Heart-blood — nothing definite. 



Culture — Same organism as above obtained from spleen. 

 Animal test — 



The spleen was rubbed into a shaved area of the abdomen of an adult 

 healthy guinea-pig weighing 520 grammes. On the third day the 

 inguinal glands could be felt to be enlarged. It steadily lost weight 

 throughout, the last weight, on the loth day, being 400 grammes. The 

 animal was then killed by chloroform, and the following appearances 

 were noted : — 



Naked-eye, — Extensive purulent ulceration of the skin limited to the 

 site of innoculation and burrowing into the subcutaneous tissue. Double 

 inguinal buboes, the surrounding blood-vessels being moderately injected. 



Spleen — somewhat enlarged with some yellow and white nodules ; a 

 few similar modules in the liver. Small intestines very hyperaemic. 



Microscopically. — No organisms were seen in the buboes or organs. 



Cultures. — The pus from the cutaneous reaction furnished a pure 

 culture of the organism. The heart-blood proved sterile. 



(291) U I 



