6 QUATERCENTENARY STUDIES IN PATHOLOGY 



Guinea-pig 5: — examined 15/9/05. 



Naked-eye appearances — 



General subcutaneous congestion ; no enlarged glands. Spleen 

 enlarged and densely studded with grey necrotic foci ; there were very 

 few in the liver also. The lungs contained a few grey nodules, each 

 surrounded by a ring of congested lung tissue. The stomach and small 

 intestines were extremely congested. 

 Microscopical examination — 



Spleen — Fairly numerous deeply stained bipolar bacilli. 



Heart-blood — a very few similar organisms. 



Cultures — 



Heart-blood — pure colonies of the organism. 

 Liver — pure colonies of the organism. 

 Spleen — pure colonies of the organism. 



Guinea-pig 6: — examined 15/9/05. 



Naked-eye appearances — 



Inguinal glands slightly enlarged. 



Spleen — somewhat enlarged. 



Liver — a few very small nodules. 



Left suprarenal capsule showed nodules, and on section was seen to 

 be almost wholly converted into cheesy masses. Lungs extremely 

 congested. Stomach and intestines apparently normal. 

 Microscopical examination — 



Nothing definite seen in heart-blood or in the organs. Suprarenal 

 capsule showed fairly numerous bipolar bacilli arranged in pairs. 



Cultures — (from heart-blood) furnished the usual growth. 



It will readily be appreciated from the details given above how 

 markedly the lesions simulate those met with in the well-known 

 pseudo-tubercle of guinea-pigs. The characters of the bacillus isolated 

 from the organs may be described briefly as corresponding closely to 

 those of B. enteritidis (Gsertner). Culturally, and in its morphology, it 

 gave evidence of being not far removed from B. coli. It can, however, 

 be readily differentiated from the colon bacillus by the fact that it does 

 not ferment lactose. It also fails to split up cane-sugar, but produces 



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