LEUCOCYTOSIS AND OPSONIC CONTENT OF SERUM II 



Regarding the leucocyte types met with in rabbit's blood, the classi- 

 fication usually adopted is as follows : — 



(i) Lymphocytes. 



(2) Large Mononuclears. 



(3) Amphophiles or Polymorphonuclears. These correspond in 



general morphology and function to the polynuclear neutro- 

 philes of man, but their granules are smaller and more ovoid, 

 and, unlike those of man, give a distinctly oxyphile reaction. 

 For the sake of convenience and uniformity of terminology, 

 we shall, in the course of this paper, refer to these cells as 

 neutrophiles, and apply the term neutrophilia to the condition 

 in which these cells are in excess. 



(4) Eosinophiles with large ovoid oxyphile granules. 



(5) Mast cells. 



As to the relative numbers of these cells in the circulating blood, 

 Lowit {loc. cit.) found 797 7o polynuclears and 20*3 % niononuclears, 

 while Hirschfeld (36) found the lymphocyte percentage equal to or even 

 greater than the polynuclear percentage. 



Schlesinger {ioc. cit.) obtained an average polynuclear percentage of 

 49, with a variation of 40 to 60. 



Brinckerhoff and Tyzzer (loc. cit.) give more complete numerical 

 relations, which will be seen to correspond closely with our own results 

 obtained from a series of 25 rabbits — 



Brinckerhoff and Tyzzer. Ledingham. 



Lymphocytes ' 45-55 per cent. 44-8 per cent. 



Large Mononuclears 2-8 w 6 n 



Amphophiles 40-50 " 42*4 n 



Eosinophiles o'5-i n it? 



Mast cells 4-8 .1 5-8 !i 



Does any marked variation in the differential count take place during 

 the day ? The question is important in view of the fact that in many of 

 our experiments the leucocyte response took the form not so much of a 

 hyperleucocytosis, as of a relative neutrophilia. It was therefore 

 important to ascertain whether control animals exhibited such variations 

 during a period of six to eight hours. 



(331) 



