l6 QUATERCENTENARY STUDIES IN PATHOLOGY 



In the following charts the polymorphonuclears are represented as "P," 

 lymphocytes as " L," mast cells as " M," large mononuclears as " L M," 

 and the eosin-nigrosinophiles as " E N." The total number of leucocytes 

 is represented as " T." " ST " and " TB " denote that the opsonic power 

 of the serum was tested against staphylococcus and tubercle bacillus 

 respectively. 



Regarding the granular series, our attention must be primarily 

 directed towards the polymorphonuclear cells. Mast cell curves and 

 eosinophile-nigrosinophile curves are also entered in the rabbit and 

 guinea-pig experiments respectively. 



The number of eosiiiophile cells in the rabbit, and of mast cells in the 

 guinea-pig, is too small, and presents too slight variations to be 

 conveniently charted. It has to be noted also that, in all cases, the 

 inoculation was made either immediately or very shortly after the first 

 sample of blood was drawn for purposes of total and differential count 

 and estimation of opsonic content. 



The quantity of sodium cinnamate (Merck) inoculated, varied from 

 •5 to I grm. 



Exp. I. (Chart I.) Rabbit. Sodium cinnamate i grm. dissolved in 

 10 cc. of sterile normal saline, inoculated intravenously. 



A very considerable hyperleucocytosis (22,900) is apparent three to 

 four hours after inoculation, and is also marked at the sixth hour. The 

 rise was due entirely to the polynuclear series, the percentage of which 

 rose from 49 before inoculation to 88 and 84 at the third and sixth hours 

 afterwards, respectively, while the non-granular cells fell from an initial 

 percentage of 40 to 10 and 14 at the same periods thereafter. 



The mast cells also fell both relatively and absolutely. 



The opsonic variation during the period under consideration was 

 scarcely appreciable. 



Exp. II. (Chart II.) Rabbit. Intravenous inoculation. 



The leucocytosis obtained was very slight at the third to the fourth 

 hour after inoculation, and at the sixth hour the blood-picture had almost 

 returned to the normal. The very considerable rise in the relative 



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