LEUCOCYTOSIS AND OPSONIC CONTENT OF SERUM 25 



The final estimation was made at the end of 120 hours, when the 

 leucocytes were found to have fallen to the normal level, and the 

 neutrophilia had completely disappeared. 



The long duration of the period of hyperleucocytosis in this 

 experiment is remarkable, and, taken in conjunction with the previous 

 experiments, would tend to show that in the guinea-pig, at least, the 

 effect of the tallianine on the haemopoietic organs is powerful and 

 prolonged. 



Remarks on Tallianine — Leucocytosis. 



The leucocytosis due to tallianine is variable, and may be entirely 

 absent when intravenous injection in rabbits is employed. 



Intraperitoneal inoculation in guinea-pigs invariably resulted in 

 marked hyperleucocytosis, which might persist over three or four days. 



Neutrophilia is invariably a conspicuous feature. We did not find so 

 rapid a response to tallianine, on the part of the white cells, as did Stas- 

 sano and Billon. As a rule the response did not make itself apparent 

 before the second or third hour. 



No injurious effects on the animals in question were ever noted. 

 ' In one rabbit experiment (intravenous inoculation of 5 cc), not 

 charted, there resulted no change whatever either in the direction of 

 hyperleucocytosis or relative neutrophilia, though estimations were made 

 hourly for six hours after inoculation. It would seem that in the rabbit, 

 at least, tallianine must disappear very rapidly from the circulation 

 after intravenous inoculation, while the absorption proceeds more slowly 

 from the guinea-pig's peritoneum. 



3. — Bouillon. 



Bouillon has long been known to produce a local leucocytosis when 

 injected into the peritoneal cavity. (Pfeiffer and Issaeff {loc. cit.) and 

 Funck (44) ). 



Exp. XV. (Chart XV.). Rabbit. Intraperitoneal injection of 

 10 cc. of a I °l^ sterile peptonised bouillon. 



Before the inoculation, the animal was found to have a very low 

 leucocyte count (4,500), but four hours afterwards it had risen to 7,350, 



(345) 



