LEUCOCYTOSIS AND OPSONIC CONTENT OF SERUM 27 



Exp. XVII. (Chart XVII.) Rabbit. Autolysed staphylococcus 



culture (2 cc.) 



A relative neutrophilia was apparent at the end of four hours, but no 

 hyperleucocytosis. At the end of 6| hours there was a very moderate 

 hyperleucocytosis of neutrophilia type, which had completely disappeared 

 at the end of 24 hours. On this occasion the opsonic index did not rise 

 at all during the first seven hours, but a sample taken at the end of 24 

 hours showed a fairly high opsonic index (1*6). 



5. — Pilocarpine. 



Ruzicka (loc. cit.) states that the immediate effect of an intravenous 

 injection of pilocarpine, in doses up to 75 mg., in the rabbit, is the 

 production of an enormous leucocytosis. In one case the leucocytes 

 rose to 235,000 almost immediately after inoculation of 50 mg. intra- 

 venously. He lays stress on the fact that the hyperleucocytosis is never 

 preceded by a period of hypoleucocytosis. 



Ruzicka's results have not been generally confirmed. Spiro (^5) 

 found an initial leucopenia with pilocarpine, followed later by a period of 

 hyperleucocytosis of neutrophile type. In the two following experiments, 

 this phenomenon was strikingly brought out. 



Exp. XVIII. (Chart XVIII.) Rabbit 60 mg. injected 

 subcutaneously. 



Ten minutes after injection the leucocytes fell from 13,400 to 10,900, 

 and one hour afterwards to 9,100. The count then commenced to rise. 

 Three hours after inoculation, the leucocytes had reached their initial 

 level, and at the sixth hour, a marked hyperleucocytosis of neutrophile 

 type was evident. 



A slight rise in the percentage of the lymphocytes during the first 

 half-hour was the only evidence of an initial mononucleosis. 



During the experiment, no appreciable change in the opsonic content 

 took place. 



Exp. XIX. (Chart XIX.) Rabbit. Intravenous inoculation (50 mg.). 



Ten minutes after injection practically no change had taken place 

 either in the total or differential count. During the next twenty minutes, 



(347) 



