LEUCOCYTOSIS AND OPSONIC CONTENT OF SERUM 33 



During the first hour there was h'ttle change in the leucocytic formula, 

 although the total count diminished. 



During the second hour, while the leucopenia persisted, the poly- 

 nuclear percentage rose considerably (69-5). At the end of the third 

 hour it had reached 70, where it remained till the end of the fifth hour. 



The total count during the latter period varied slightly, but there was 

 no distinct period of hyperleucocytosis. At the end of 24 hours the 

 leucocytic formula had returned to the normal. 



The opsonic index exhibited a marked negative phase during the 

 first hour after inoculation, and was succeeded by a moderate rise during 

 the second hour. The maximum opsonic power, both to staphylococcus 

 and tubercle, was registered in the course of the third hour, but was 

 exceedingly transitory. It fell to normal again in the fourth and fifth 

 hours. 



The negative phase in the case of tubercle was somewhat more 

 pronounced than in the case of staphylococcus. It is notable that the 

 high opsonic level coincided fairly exactly with the attainment of the 

 high neutrophilic phase, but this latter persisted much longer. 



Exp. XXIV. (Chart XXIV.) Guinea-pig. Intraperitoneal 

 inoculation (i cc). 



A well marked hyperleucocytosis of neutrophilic type took place, and 

 the opsonic index rose to double its original value in four hours. 



Exp. XXV. (Chart XXV.) Rabbit, (i cc). 



Four hours after inoculation, the leucocytes showed a very slight rise, 

 v/hich was accompanied by an enormous neutrophilia (80 7o)- The 

 opsonic index to staphylococcus also rose in four hours to i'6. 



Exp. XXVI. (Chart XXVI.) Rabbit, (i cc). 



The inoculation was made at 5-30 p.m., but by next morning, any 

 effect that the nuclein may have had on the leucocytes and opsonic 

 content had completely disappeared. Accordingly, another inoculation 

 was given at 10*45 a.m. A sample drawn at 5*15 p.m. on the same day 



(353) Y 



