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PROPERTIES OF ILEMOLYTIC SERA 



EXAMPLE 3. Details as before. 



SERIES (a). 1 c.c. of 5 per cent, suspension of corpuscles and 

 1 dose of immune-body. 



SERIES (6). 1 c.c. of suspension of corpuscles and 7 doses 

 of immune-body. 



M.H.D. of complement = 0*017 c.c. 



Result. 1 dose of immune-body takes up 0-035 c.c. of complement. 

 7 doses of immune-body take up 0-21 c.c. of complement. 



EXAMPLE 4. Details as before. Here only the results need be given. 

 2 doses of I B took up 0-04 c.c. of guinea-pig's complement. 

 11 0-195 c.c. 



13 0-22 c.c. 



M.H.D. of complement = 0-015 c.c. 



Numerous other experiments have given corresponding 

 results, and as much as twenty or even more doses of com- 

 plement may be taken up when high multiples of immune- 

 body are used. 



The general result is that up to a certain point each dose 

 of immune-body leads to the taking up of additional com- 

 plement, and if a few, say three or four, doses of immune- 

 body are present the amount of complement combined 

 is almost proportionate. With a greater number of doses, 

 however, the amount is proportionately not quite so great. 

 We have to bear in mind, however, that all the immune- 

 body added does not enter into combination, a fraction 

 remaining free in the fluid, and this naturally will not lead 

 to the union of complement. With eight doses of immune- 

 body, for example, nearly one dose remains free (vide supra) ; 



